Penentuan kadar katekin ekstrak air daun dan kulit batang faloak dengan metode spektrofotometri UV-Vis
DOI: https://doi.org/10.56922/pti.v6i5.4036
Catechin Faloak Spectrophotometry UV-Vis
Abstract
Background: Faloak (Sterculia quadrifida R.Br.) is a medicinal plant native to East Nusa Tenggara that contains catechin as one of its bioactive compounds. Catechin content may be influenced by plant parts and environmental factors, including altitude.
Purpose: This study aimed to determine and compare the catechin content of aqueous extracts from the leaves and stem bark of faloak collected from different altitudes.
Method: Leaf and stem bark samples of faloak were collected from Bolok (30 m above sea level) and Camplong (320 m above sea level) and extracted using the infusion method. Catechin content was determined by uv-visible spectrophotometry at a wavelength of 288 nm using (+)-catechin as the standard.
Results: The highest catechin content was found in the aqueous stem bark extract from Camplong (3.27 ± 0.04 mg/g), while the lowest was observed in the aqueous leaf extract from Bolok (0.77 ± 0.07 mg/g).
Conclusion: Catechin content in faloak is influenced by plant parts and altitude. The stem bark contained higher catechin levels than the leaves, and samples collected from higher altitudes exhibited greater catechin content than those from lower altitudes.
Keywords: Catechin; Faloak; Uv-vis Spectrophotometry.
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