Profil kromatogram ekstrak etanol 95% dan etil asetat jahe merah (Zingiber officinale var. rubrum)
DOI: https://doi.org/10.56922/pti.v6i4.3962
HPLC Red ginger Zingiber officinale var. Rubrum
Abstract
Background: Red ginger (Zingiber officinale var. Rubrum) is rich in bioactive compounds, such as gingerol and shogaol, which are highly sensitive to type of extraction solvent. Therefore, a chromatogram profile analysis is required to determine most effective solvent.
Purpose: This study aims to compare chromatogram profiles of 95% ethanol and ethyl acetate extracts of red ginger rhizome using High-Performance Liquid Chromatography (HPLC).
Method: This descriptive laboratory study extracted 90 gr of red ginger crude drug (simplisia) powder from Ende Regency, Flores, via maceration using 95% ethanol and ethyl acetate for 3 × 24 hours. Resulting filtrates were evaporated to obtain thick extracts, which were then analyzed using HPLC to evaluate the number of peaks, retention time, and peak area.
Results: 95% ethanol extract produced a more complex chromatogram profile with 30 peaks, whereas the ethyl acetate extract yielded 22 peaks. This difference indicates superiority of 95% ethanol in extracting secondary metabolite compounds across a wider polarity range compared to ethyl acetate.
Conclusion: There is a significant difference in chromatogram profiles between two extracts. 95% ethanol solvent proved to be more effective in extracting bioactive compounds from red ginger rhizome than ethyl acetate, serving as a baseline for herbal raw material standardization and a foundation for future research.
Keywords: HPLC; Red ginger; Zingiber officinale var. Rubrum.
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