Pengaruh pemberian serbuk daun salam (Syzygium polyanthum) terhadap kadar hemoglobin pada tikus wistar model anemia
DOI: https://doi.org/10.33024/hjk.v20i7.3716
Anemia Hemoglobin Rattus norvegicus Syzygium polyanthum Wistar Rats
Abstract
Background: Anemia remains a major global public health problem, particularly among women of reproductive age. The prevalence of anemia in this population ranges from 27% to 32.8%. In Indonesia, anemia prevalence remains high, affecting 48.9% of adolescent girls and 46.9% of pregnant women. Iron deficiency due to inadequate dietary iron intake and impaired iron absorption is the primary cause of anemia. Indonesian bay leaf (Syzygium polyanthum) powder is a locally available food resource containing iron and has the potential to be developed as an alternative nutritional intervention to improve hemoglobin levels.
Purpose: To evaluate the effect of Syzygium polyanthum leaf powder supplementation on hemoglobin levels in anemic female Wistar rats.
Method: A pretest–posttest controlled experimental design conducted at the Integrated Research and Testing Laboratory, Universitas Gadjah Mada, and the Integrated Laboratory of Universitas Sebelas Maret from August to October 2023. Six-week-old female Wistar rats (Rattus norvegicus) weighing approximately 150 g were obtained from UD Wistar, Yogyakarta. During the acclimatization period, the rats were fed commercial feed and an iron-deficient AIN-93M diet. Anemia was induced by administering an iron-deficient diet for 15 days. Subsequently, Syzygium polyanthum leaf powder was orally administered for 28 days at three different doses: P1 (18.6 mg/200 g body weight), P2 (36 mg/200 g body weight), and P3 (55.6 mg/200 g body weight). Hemoglobin levels were measured using the cyanmethemoglobin method.
Results: Administration of Syzygium polyanthum leaf powder for 28 days significantly increased hemoglobin levels. The greatest improvement was observed in the P1 group (18.6 mg/200 g body weight), with a mean hemoglobin increase of 1.16 ± 1.34 g/dL.
Conclusion: Supplementation with Syzygium polyanthum leaf powder at a dose of 18.6 mg/200 g body weight significantly improved hemoglobin levels in anemic female Wistar rats. Syzygium polyanthum leaf powder has potential as an alternative intervention for anemia; however, further studies are required to confirm its effectiveness.
Keywords: Anemia; Hemoglobin; Rattus norvegicus; Syzygium polyanthum; Wistar Rats.
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Adyani, K., Anwar, A. D., & Rohmawaty, E. (2018). Peningkatan kadar hemoglobin dengan pemberian ekstrak daun salam (Syzygium Polyanthum (Wight) Walp) pada tikus model anemia defisiensi besi. Majalah Kedokteran Bandung, 50(3), 167-172.
Bahriul, P., Rahman, N., & Diah, A. W. M. (2014). Uji aktivitas antioksidan ekstrak daun salam (Syzygium polyanthum) dengan menggunakan 1, 1-difenil-2-pikrilhidrazil. Jurnal Akademika Kimia, 3(3), 143-149.
Basir, H. (2018). Penetapan Kadar Vitamin C Pada Daun Salam (Syzygium Polyanthum) Secara Iodimetri. Jurnal Kesehatan Yamasi Makassar, 2(1).
Bhadreswara, I. G. R. W., & Susanti, N. M. P. (2023). Potensi Daun Salam (Syzygium Polyanthum) Sebagai Antioksidan Untuk Menangkal Radikal Bebas. In Prosiding Workshop Dan Seminar Nasional Farmasi (Vol. 2, pp. 620-630).
Cappellini, M. D., & Motta, I. (2015). Anemia in clinical practice—definition and classification: does hemoglobin change with aging?. In Seminars in hematology (Vol. 52, No. 4, pp. 261-269). WB Saunders.
Longo, D. L., & Camaschella, C. (2015). Iron-deficiency anemia. N Engl J Med, 372(19), 1832-43.
Cappellini, M. D., Musallam, K. M., & Taher, A. T. (2020). Iron deficiency anaemia revisited. Journal of internal medicine, 287(2), 153-170.
Devi, R. M., Nuhriawangsa, A. M. P., & Rahardjo, S. S. (2024). Pengaruh pemberian serbuk daun salam (Syzygium polyanthum) terhadap kadar feritin pada tikus wistar model anemia. Media Penelitian dan Pengembangan Kesehatan, 34(3), 757-766.
Devi, R. M., Nuhriawangsa, A. M. P., & Rahardjo, S. S. (2023). Syzygium Polyanthum as a Potential Hemoglobin Booster for Iron Deficiency Anemia Due to its High Vitamin C Content. In Proceedings The International Allied Health Students Conference (IAHSC) (pp. 167-170).
Fertrin, K. Y. (2020). Diagnosis and management of iron deficiency in chronic inflammatory conditions (CIC): is too little iron making your patient sick?. Hematology 2014, the American Society of Hematology Education Program Book, 2020(1), 478-486.
Finazzi, D., & Arosio, P. (2014). Biology of ferritin in mammals: an update on iron storage, oxidative damage and neurodegeneration. Archives of toxicology, 88(10), 1787-1802.
Hedayanti, N., & Wahyudi, A. (2023). Review of Physiological Aspects of Erythropoie sis: A Narrative Literature Review. Sriwijaya Journal of Internal Medicine, 1(1), 18-24.
Ismail, A., & Ahmad, W. A. N. W. (2019). Syzygium polyanthum (Wight) Walp: A potential phytomedicine. Pharmacognosy Journal, 11(2), 429-438.
Kementerian Kesehatan Republik Indonesia. (2020). Pedoman penatalaksanaan pemberian tablet tambah darah (TTD) bagi remaja putri pada masa pandemi COVID-19. Diakses dari: https://ayosehat.kemkes.go.id/buku-pedoman-penatalaksanaan-pemberian-tablet-tambah-darah
Kementerian Kesehatan Republik Indonesia. (2019). Laporan nasional Riskesdas 2018. Diakses dari: https://repository.kemkes.go.id/book/1323
Komaria, R., Lister, I. N. E., & Lie, S. (2021, July). Hematological Profiles of Benzene-Induced Rats Treated with Ethanolic Extract of Syzygium Polyanthum. In 2021 IEEE International Conference on Health, Instrumentation & Measurement, and Natural Sciences (InHeNce) (pp. 1-6). IEEE.
Mantadakis, E., Chatzimichael, E., & Zikidou, P. (2020). Iron deficiency anemia in children residing in high and low-income countries: risk factors, prevention, diagnosis and therapy. Mediterranean journal of hematology and infectious diseases, 12(1), e2020041.
Najib, A., Olli, A. T., & Puspitasari, Y. (2025). Optimasi Ekstrak Daun Salam (Syzygium polyanthum) Menggunakan Metode Konvensional Dan Green Extraction Serta Profil Kimia Dan Potensi Antioksidannya. Jurnal Mandala Pharmacon Indonesia, 11(1), 55-65.
Novira, P. P., & Febrina, E. (2018). Review Artikel: Tinjauan aktivitas farmakologi ekstrak daun salam (Syzygium polyanthum (Wight.) Walp). Farmaka, 16(2).
Osterholm, E. A., & Georgieff, M. K. (2015). Chronic inflammation and iron metabolism. The Journal of pediatrics, 166(6), 1351.
Ratih, G. A. M., Aryastuti, A. A. S. A., Suryaningsih, P. A., Agustini, D. G. D., & Habibah, N. (2025). Hematopoietic Effects Of Ethanolic Extract From Alternanthera Sissoo On Erythrocyte, Hemoglobin, And Hematocrit Levels In Anemia-Induced Female Wistar Rats. Meditory: The Journal of Medical Laboratory, 13(2), 143-153.
Suharni, S., Indarto, D., & Cilmiaty, R. (2021). Comparison of ethanol and methanol extracts of bay leaves (Syzygium polyanthum) in terms of vitamin c, iron and phytochemical levels. In Proceedings of the 1st International Seminar on Teacher Training and Education (pp. 3-8).
Sumiwi, S. A., Zuhrotun, A., Hendriani, R., Rizal, M., Levita, J., & Megantara, S. (2019). Subchronic toxicity of ethanol extract of Syzygium polyanthum (Wight) Walp. leaves on wistar rat. The Indonesian Biomedical Journal, 11(1), 30-5.
Suprapti, E., Hadju, V., Ibrahim, E., Indriasari, R., Erika, K. A., & Balqis, B. (2025). Anemia: etiology, pathophysiology, impact, and prevention: a review. Iranian journal of public health, 54(3), 509.
World Health Organization. (2022). Prevalence of anaemia in women aged 15 to 49 (%). Diakses dari: https://data.who.int/indicators/i/41D099F/8D58801
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