Efektivitas pemberian topikal gel ekstrak nanas (Ananas Comosus) terhadap kolagen pada tikus wistar (Rattus Norvegicus) dengan luka bakar grade 2 studi pada penyembuhan luka epidermis, kadar IL-1, dan biomarker kolagen
DOI: https://doi.org/10.33024/hjk.v20i6.3550
Epidermal Wound Healing Grade II Burn Interleukin-1 (IL-1) Pineapple Extract (Ananas Comosus)
Abstract
Background: Grade II burn injury is a tissue injury involving the epidermis and part of the dermis, requiring an optimal healing process. Pineapple (Ananas comosus) contains the enzyme bromelain, which has anti-inflammatory effects and the potential to accelerate wound healing by reducing pro-inflammatory cytokine levels and increasing collagen synthesis.
Purpose: To analyze the effectiveness of the topical application of pineapple extract (Ananas comosus) gel on grade II burn wound healing in Wistar rats (Rattus norvegicus), based on wound diameter, Interleukin-1 levels, and collagen deposition parameters.
Method: An experimental study with a post-test-only control group design using 30 male Wistar rats (Rattus norvegicus). Sampels were divided into six groups: normal control group (KN), negative control group (K-), positive control group (K+), and treatment groups with pineapple extract gel at concentrations of P1 (15%), P2 (20%), and P3 (25%). Grade II burns injuries were induced on the rats' backs, and topical therapy was administered twice daily for 14 days. Parameters observed included wound diameter, IL-1 levels, and collagen. Data were analyzed using the Kruskal Wallis test and One-Way ANOVA.
Results: There were significant differences in wound diameter (p = 0.004), IL-1 levels (p < 0.001), and collagen (p < 0.001) among the groups. Group P2 (20%) showed the smallest wound diameter, whereas Group P3 (25%) showed the lowest IL-1 levels and the highest collagen levels.
Conclusion: Topical application of pineapple extract gel is effective in accelerating grade II burn wound healing through mechanisms of inflammation reduction and increased collagen formation. A concentration of 20% is optimal for clinical wound healing, while 25% is optimal for improving tissue quality histologically.
Suggestion: Further studies are needed to examine the specific molecular mechanisms of pineapple extract gel, evaluate long term toxicity tests, and conduct human clinical trials to assess its efficacy and safety as a topical therapy for grade II burns.
Keywords: Epidermal Wound Healing; Grade II Burn; Interleukin-1 (IL-1); Pineapple Extract (Ananas Comosus).
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References
Abdullahi, A., Amini-Nik, S., & Jeschke, M. G. (2014). Animal models in burn research. Cellular and Molecular Life Sciences, 71(17), 3241-3255.
Atiyeh, B. S., Hayek, S. N., & Gunn, S. W. (2005). New technologies for burn wound closure and healing review of the literature. Burns, 31(8), 944–956.
Badriyya, E., Pratiwi, A. R., Dillasamola, D., Aldi, Y., & Husni, E. (2020). Topical anti-inflammatory activity of bromelain. Pharmacogn. J, 12, 1586.
Beserra, F. P., Gushiken, L. F. S., Vieira, A. J., Bérgamo, D. A., Bérgamo, P. L., Souza, M. O. D., & Pellizzon, C. H. (2020). From inflammation to cutaneous repair: topical application of lupeol improves skin wound healing in rats by modulating the cytokine levels, nf-κb, ki-67, growth factor expression, and distribution of collagen fibers. International Journal of Molecular Sciences, 21(14), 4952.
Bhattacharyya, B. K. (2008). Bromelain: an overview. Natural Product Radiance, 7(4), 359–363.
Christanto, A., Cilmiaty, R., Setiamika, M., Yudhani, R. D., Dirgahayu, P., Pamungkasari, E. P., & Nurwati, I. (2025). The role of interleukin 1β, fibroblast growth factor, fibroblasts, keratinocytes, granulation tissue and collagen density in the wound healing phase (study of wound healing in the inflammation, proliferation, and remodeling phases). Biocaster: Jurnal Kajian Biologi, 5(3), 108-118.
Fathi, A. N., Babaei, S., Babaei, S., Baazm, M., Sakhaie, H., & Babaei, S. (2022). Effect of bromelain on mast cell numbers and degranulation in diabetic rat wound healing. Journal of Wound Care, 31(Sup8), S4-S11.
Ivanko, A., Garbuzov, A. E., Schoen, J. E., Kearns, R., Phillips, B., Murata, E., & Carter, J. E. (2024). The burden of burns: an analysis of public health measures. Journal of Burn Care & Research, 45(5), 1095-1097.
Jeschke, M. G., van Baar, M. E., Choudhry, M. A., Chung, K. K., Gibran, N. S., & Logsetty, S. (2020). Burn injury. Nature Reviews Disease Primers, 6(1), 11.
Kang, S., Amagai, M., & Bruckner, A. L. (2019). Fitzpatrick’s dermatology 9 th edition. New York: Mc Graw Hill Education. p. 1679–1690.
Katz, S. I., Gilchrest, B. A., Paller, A. S., & Leffell, D. J. (2008). Fitzpatrick's dermatology in general medicine (pp. 690-703). K. Wolff, & L. A. Goldsmith (Eds.). New York: McGraw-Hill.
Korkmaz, H. I., Flokstra, G., Waasdorp, M., Pijpe, A., Papendorp, S. G., de Jong, E., & van Zuijlen, P. P. M. (2023). The complexity of the post-burn immune response: an overview of the associated local and systemic complications. Cells, 12(3), 345.
Lestari, T., Syukur, S., Revilla, G., Sukma Rita, R., & Rustini, R. (2023). The burn wound healing process: a review. International Journal of Progressive Sciences and Technologies, 40(1), 77-88.
Masbagusdanta, K., Setiasih, S., Handayani, S., & Hudiyono, S. (2020). Partial purification and evaluation of bromelain from pineapple stem (ananas comosus) in cream based preparation and its in vitro anti-inflammatory activity. AIP Conference Proceedings 2243 (1, p. 030012).
Maurer, H. R. (2001). Bromelain: biochemistry, pharmacology and medical use. Cellular and Molecular Life Sciences, 58, 1234–1245.
Pavan, R., Jain, S., Shraddha, & Kumar, A. (2012). Properties and therapeutic application of bromelain: a review. Biotechnology Research International, 2012(1), 976203.
Purmawanti, S. (2024). Pengaruh krim ekstrak daun pegagan (centella asiatica) terhadap inhibisi mmp-1 dan peningkatan kolagen (studi eksperimental pada mencit yang dipapar sinar uvb akut) (Master's thesis, Universitas Islam Sultan Agung (Indonesia).
Ramli, R. N., Prawoto, A., Riasa, N. P., Saputro, I. D., & Mas' Ud, A. F. (2021). Epidemiology and knowledge of first aid treatment related to burn injury in the rural region of kulon progo, indonesia. Open Access Macedonian Journal Medical Sciences, 9 (E): 101-108.
Rodrigues, M., Kosaric, N., Bonham, C. A., & Gurtner, G. C. (2018). Wound healing: a cellular perspective. Physiological Reviews, 99(1), 665–706.
Schaefer, T. J., & Szymanski, K. D. (2017). Burn evaluation and management.
Tan, J. L., Lash, B., Karami, R., Nayer, B., Lu, Y. Z., Piotto, C., & Martino, M. M. (2021). Restoration of the healing microenvironment in diabetic wounds with matrix-binding il-1 receptor antagonist. Communications biology, 4(1), 422.
Waladani, B., Ernawati, E., & Suwaryo, P. A. W. (2021). Peningkatan pengetahuan dan keterampilan kader kesehatan masyarakat dalam pertolongan pertama dengan kasus luka bakar. Jurnal Peduli Masyarakat, 3(2), 185-192.
Weinheimer-Haus, E. M., Mirza, R. E., & Koh, T. J. (2015). Nod-like receptor protein-3 inflammasome plays an important role during early stages of wound healing. PLoS One, 10(3), e0119106.
Żwierełło, W., Piorun, K., Majewicz, M. S., Maruszewska, A., Antoniewski, J., & Gutowska, I. (2023). Burns: classification, pathophysiology, and treatment: a review. International Journal Of Molecular Sciences, 24(4), 3749.
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