Profil imunoglobulin-g dan imunoglobulin-m pasien suspek demam berdarah dengue
DOI: https://doi.org/10.33024/hjk.v20i2.2339
Demam Berdarah Dengue Imunoglobulin-M Pasien Suspek Profil Imunoglobulin-G
Abstract
Background: Limited access to and affordability of standard dengue diagnostic tests in primary healthcare facilities in Indonesia result in many cases remaining unconfirmed by laboratory examination. Therefore, routine hematological parameters may serve as a more affordable alternative for early dengue diagnosis, particularly in resource-limited settings.
Purpose: To assess the association and diagnostic accuracy of routine hematological parameters with dengue diagnosis and infection status (primary or secondary) as determined by IgM and IgG serological profiles.
Method: A quantitative study using a cross-sectional approach was conducted from January to December 2024 at the Laboratory of the Pancaran Kasih Evangelical Church General Hospital in Manado. The sampling technique used purposive sampling with a sample size of 141 respondents. The independent variable in this study was suspected dengue fever status, while the dependent variables were the immunoglobulin G (IgG) and immunoglobulin M (IgM) profiles of the patients. Data analysis was performed using R software with a significance level of 0.05.
Results: Platelet count and the hemoconcentration/thrombocytopenia index (HTI) showed the best performance in distinguishing dengue-positive from dengue-negative cases (AUC 0.675), while leukocyte count demonstrated good discriminative ability in differentiating primary and secondary infections (AUC 0.817).
Conclusion: Platelet count and the hemoconcentration/thrombocytopenia index (HTI) are useful as adjunct tools for early dengue diagnosis, while leukocyte count is the best indicator for differentiating primary and secondary infections and risk stratification.
Keywords: Dengue Fever; Immunoglobulin-G Profile; Immunoglobulin-M; Suspected Patients
Pendahuluan: Keterbatasan dan keterjangkauan akses pemeriksaan diagnostik standar dengue di fasilitas kesehatan tingkat pertama di Indonesia menyebabkan banyak kasus tidak terkonfirmasi secara laboratorik. Oleh karena itu, parameter hematologi rutin berpotensi dimanfaatkan sebagai alternatif diagnostik awal yang lebih terjangkau, khususnya di wilayah dengan sumber daya terbatas.
Tujuan: Untuk menilai hubungan dan akurasi diagnostik antara parameter hematologi rutin dengan diagnosis DBD dan status infeksi (primer atau sekunder) yang ditetapkan melalui pemeriksaan profil serologis IgM dan IgG.
Metode: Penelitian kuantitatif dengan pendekatan cross sectional, dilaksanakan pada bulan Januari-Desember 2024, di Laboratorium Rumah Sakit Umum Gereja Masehi Injili Pancaran Kasih, Manado. Teknik pengambilan sampel menggunakan purposive sampling dengan jumlah sampel yang digunakan sebanyak 141 responden. Variabel independen dalam penelitian ini adalah status suspek demam berdarah dengue, sedangkan variabel dependen adalah profil imunoglobulin G (IgG) dan imunoglobulin M (IgM) pasien. Analisis data dilakukan menggunakan perangkat lunak R dengan tingkat signifikansi 0.05.
Hasil: Trombosit dan indeks hemokonsentrasi/trombositopenia (HTI) memiliki kinerja terbaik untuk membedakan dengue positif dan negatif dengan akurasi (AUC 0.675), leukosit menunjukkan kemampuan diskriminasi yang baik dalam membedakan infeksi primer dan sekunder (AUC 0.817).
Simpulan: Trombosit dan indeks hemokonsentrasi/trombositopenia (HTI), bermanfaat sebagai alat bantu diagnosis awal dengue, sementara leukosit merupakan indikator terbaik untuk membedakan infeksi primer dan sekunder serta stratifikasi risiko.
Kata Kunci: Demam Berdarah Dengue; Imunoglobulin-M; Pasien Suspek; Profil Imunoglobulin-G.
Downloads
References
Asish, P. R., Rachel, G., Sindhuja, V., Pradhan, J., Dasgupta, S., & Chethrapilly Purushothaman, G. K. (2025). Differentiating Secondary Dengue Cases Using Capture Igg And Igm-Based Approaches. Journal Of Pure & Applied Microbiology, 19(2).
Chaloemwong, J., Tantiworawit, A., Rattanathammethee, T., Hantrakool, S., Chai-Adisaksopha, C., Rattarittamrong, E., & Norasetthada, L. (2018). Useful Clinical Features And Hematological Parameters For The Diagnosis Of Dengue Infection In Patients With Acute Febrile Illness: A Retrospective Study. BMC Hematology, 18(1), 20.
Gupta, B. P., Uranw, S., Gupta, V. P., Deuba, E., Sah, A. K., Chaudhary, S., & Wagle, C. (2025). Leukopenia And Thrombocytopenia In Dengue Patients: A Cross-Sectional Study From A Tertiary Hospitals In Koshi Province, Nepal. BMC Infectious Diseases, 25(1), 753.
Guzman, M. G., Gubler, D. J., Izquierdo, A., Martinez, E., & Halstead, S. B. (2016). Dengue Infection. Nature Reviews Disease Primers, 2(1), 16055.
Haider, M., Yousaf, S., Zaib, A., Sarfraz, A., Sarfraz, Z., & Cherrez-Ojeda, I. (2022). Diagnostic Accuracy Of Various Immunochromatographic Tests For NS1 Antigen And Igm Antibodies Detection In Acute Dengue Virus Infection. International Journal Of Environmental Research And Public Health, 19(14), 8756.
Haq, F. U., Imran, M., Aslam, Z., Mukhtar, F., Jabeen, K., Chaudhry, M., & Muhammad, N. (2023). Severity Of Dengue Viral Infection Based On Clinical And Hematological Parameters Among Pakistani Patients. The American Journal Of Tropical Medicine And Hygiene, 109(6), 1284.
Harapan, H., Michie, A., Mudatsir, M., Sasmono, R. T., & Imrie, A. (2019). Epidemiology Of Dengue Hemorrhagic Fever In Indonesia: Analysis Of Five Decades Data From The National Disease Surveillance. BMC Research Notes, 12(1), 350.
Iryanti, M. P., Raharjo, M., Martini, M., Setiani, O., & Joko, T. (2025). Spatio-Temporal Analysis Of DHF Incidence With Environmental Factors In Batam City, Indonesia. Universal Journal Of Public Health, 13(1), 33-53.
Kalra, C., Mittal, G., Gupta, P., Agarwal, R. K., Ahmad, S., & Kumar Agarwal, R. (2024). Role Of Igm/Igg Ratio In Distinguishing Primary And Secondary Dengue Viral Infections: A Cross-Sectional Study. Cureus, 16(8).
Karyanti, M. R., Uiterwaal, C. S., Kusriastuti, R., Hadinegoro, S. R., Rovers, M. M., Heesterbeek, H., & Bruijning-Verhagen, P. (2014). The Changing Incidence Of Dengue Haemorrhagic Fever In Indonesia: A 45-Year Registry-Based Analysis. BMC Infectious Diseases, 14(1), 412.
Kharisma, S. B. (2024). Gambaran Hasil Pemeriksaan Igg Dan Igm Pada Pasien Demam Berdarah Dengue Di Rumah Sakit Islam Jombang (Doctoral Dissertation, Itskes Insan Cendekia Medika Jombang).
Pillay, K., Keddie, S. H., Fitchett, E., Akinde, C., Bärenbold, O., Bradley, J., & Dubot-Pérès, A. (2025). Evaluating The Performance Of Common Reference Laboratory Tests For Acute Dengue Diagnosis: A Systematic Review And Meta-Analysis Of RT-PCR, NS1 Elisa, And Igm Elisa. The Lancet Microbe.
Pramesti, S. R., Hartini, S., Prihandono, D. S., & Wahyunie, S. (2024). Overview Of Immunoglobulin M (Igm) And Immunoglobulin G (Igg) Examination Results In Children With Dengue Fever At Inche Abdoel Moeis Regional Hospital In 2023. MMLTJ (Mahakam Medical Laboratory Technology Journal), 4(2), 86-95.
Putri, I. G. A. A. E., Aryati, A., Hakim, F. K. N., Tanzilia, M. F., Zuroidah, N., Wrahatnala, B. J., & Miftahussurur, M. (2023). Non-Structural Protein 1 And Hematology Parameters As Predictors Of Dengue Virus Infection Severity In Indonesia. Journal Of Medicine And Life, 16(10), 1546.
Rahmawati, L. N. (2016). Gambaran Imunoglobulin G (Igg) Pada Pasien Post Demam Berdarah Dengue (Dbd)(Studi Di Puskesmas Mojowarno) (Doctoral Dissertation, Stikes Insan Cendekia Medika Jombang).
Rodrigo, C., Sigera, C., Fernando, D., & Rajapakse, S. (2021). Plasma Leakage In Dengue: A Systematic Review Of Prospective Observational Studies. BMC Infectious Diseases, 21(1), 1082.
Sasmono, R. T., Taurel, A. F., Prayitno, A., Sitompul, H., Yohan, B., Hayati, R. F., & Nealon, J. (2018). Dengue Virus Serotype Distribution Based On Serological Evidence In Pediatric Urban Population In Indonesia. Plos Neglected Tropical Diseases, 12(6), E0006616.
Thapa, B., Lamichhane, P., Shrestha, T., Lamichhane, S., Karki, S., Pradhananga, S., & Pudasaini, P. (2025). Leukopenia And Thrombocytopenia In Dengue Patients Presenting In The Emergency Department Of A Tertiary Center In Nepal: A Cross-Sectional Study. BMC Infectious Diseases, 25(1), 56.
Utama, I. M. S., Lukman, N., Sukmawati, D. D., Alisjahbana, B., Alam, A., Murniati, D., & Parwati, K. T. M. (2019). Dengue Viral Infection In Indonesia: Epidemiology, Diagnostic Challenges, And Mutations From An Observational Cohort Study. Plos Neglected Tropical Diseases, 13(10), E0007785.
Yunarsih, Y., Haryanto, E., & Rahayuningsih, C. K. (2025). Keterkaitan Jumlah Trombosit Dengan Imunoglobulin G Dan Jumlah Lekosit Dengan Imunoglobulin M Pada Kasus Demam Berdarah Dengue. Jurnal Penelitian Kesehatan" Suara Forikes"(Journal Of Health Research" Forikes Voice"), 16(3), 781-785.
Downloads
Published
Issue
Section
License
Copyright (c) 2026 Holistik Jurnal Kesehatan
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
